ADH5 Antikörper (Internal Region)

Produktnummer ABIN374869

Dieser Ziege Polyklonal Antikörper detektiert spezifisch ADH5 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Rind (Kuh) und Hund.

Kurzübersicht für ADH5 Antikörper (Internal Region) (ABIN374869)

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ADH5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ADH5 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-KKIKVDEFVT HN

Spezifität: This antibody detects Alcohol Dehydrogenase 5 (ADH5).

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Andere Bezeichnung: Alcohol Dehydrogenase 5 (ADH5)

Hintergrund: The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Molekulargewicht: 39.7 kDa according to NP_000662.3.

Gen-ID: 128, 9606

UniProt: P11766