SMN1 / SMN2 (all Isoforms), (Internal Region) Antikörper

Produktnummer ABIN374850

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in WB. Geeignet für Human.

Kurzübersicht für SMN1 / SMN2 (all Isoforms), (Internal Region) Antikörper (ABIN374850)

Target: SMN1 / SMN2

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Bindungsspezifität: all Isoforms, Internal Region

Sequenz: C-DESENSRSPG NKSDN

Spezifität: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to internal region of Human SMN1+SMN2 according to NP_075012.1, NP_000335.1, NP_075013.1, NP_075014.1, NP_075015.1, NP_059107.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 28.6 kDa according to NP_075012.1 and NP_075014.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: SMN1 / SMN2

Hintergrund: The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly.Synonyms: Component of gems 1, Gemin-1, Gemin1, SMN, SMNC, SMNT, Survival motor neuron protein

Gen-ID: 6606

NCBI Accession: NP_000335

UniProt: Q16637