ABHD5 Antikörper (Internal Region)

Produktnummer ABIN374842

Dieser Ziege Polyklonal Antikörper detektiert spezifisch ABHD5 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Hund.

Kurzübersicht für ABHD5 Antikörper (Internal Region) (ABIN374842)

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ABHD5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ABHD5 Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody recognizes CGI58 / ABHD5. AP16647PU-N is not expected to cross-react with ABHD4.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence Genename: ABHD5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: ABHD5 Antibody detection limit dilution: 1/16000. Western Blot: 0.2-0.6 μg/mL. Approx 40 kDa band observed in lysates of cell lines NIH3T3and A431 calculated MW of 39.0 kDa according to NP_057090.2). Additional faint bands of20 kDa and 10 kDa were observed. These bands were successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ABHD5

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Andere Bezeichnung: ABHD5

Hintergrund: ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. ABHD5 is widely expressed in various tissues, including skin, lymphocytes, liver, skeletal muscle and brain.Synonyms: Abhydrolase domain-containing protein 5, CGI-58, NCIE2

Gen-ID: 51099, 9606

UniProt: Q8WTS1

Pathways: Lipid Metabolism