DKC1 Antikörper (Internal Region)

Produktnummer ABIN374810

Der Ziege Polyklonal Anti-DKC1-Antikörper wurde für IHC (p) validiert. Er ist geeignet, DKC1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für DKC1 Antikörper (Internal Region) (ABIN374810)

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DKC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DKC1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-KRKRESESES DETPP

Spezifität: This antibody detects Dyskerin.

Kreuzreaktivität (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001354.1 Genename: DKC1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: Preliminary experiments gave an approx 70-75 kDa band in Human BoneMarrow, Duodenum and Skin lysates after 0.01 μg/mL antibody staining. Please note thatcurrently we cannot find an explanation in the literature for the band we observe given thecalculated size of 57.5 kDa according to NP_001354.1. The 70-75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Spleen shows strong nuclear

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu DKC1

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Andere Bezeichnung: DKC1 / NOLA4

Hintergrund: DKC1 (Dyskeratosis congenita 1) is a nucleolar protein, altered in dyskeratosis congenita, which carries out two separate functions, both fundamental for proliferating cells. One function is the pseudouridylation of ribosomal RNA (rRNA) molecules, necessary for their processing, and the other is the stabilization of the telomerase RNA component, necessary for telomerase activity. It is thought to be the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. DKC1 is also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Both 18S rRNA production and rRNA pseudouridylation are impaired if DKC1 is depleted.Synonyms: CBF5 homolog, Dyskerin, H/ACA ribonucleoprotein complex subunit 4, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1

Gen-ID: 1736

NCBI Accession: NP_001354

UniProt: O60832

Pathways: Telomere Maintenance