Nanog Antikörper (C-Term)

Produktnummer ABIN374808

Produktdetails anti-Nanog Antikörper

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-DLFLNYSMNM QPED

Spezifität: Recognizes Human NANOG (C-term). Others species not tesetd.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_079141.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000. Western blot: 0.05-0.1 μg/mL. Approx 35 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 34.5 kDa according to NP_079141.1). An additional band of approx.35 kDa is observed and is consistent with theobservations of higher molecular weight bands regularly found with antibodies from othersources. Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows nuclear staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG (NANOG Produkte)

Synonyme: 2410002E02Rik antikoerper, ENK antikoerper, ecat4 antikoerper, NANOG antikoerper, hacp antikoerper, wu:fd19e04 antikoerper, wu:fd20a07 antikoerper, zgc:193933 antikoerper, Nanog homeobox antikoerper, nanog homeobox antikoerper, Nanog antikoerper, NANOG antikoerper, nanog antikoerper

Hintergrund: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

Gen-ID: 79923, 9606

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance