Iba1 Antikörper (C-Term, Isoform 1)

Produktnummer ABIN374779

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Iba1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Hund.

Kurzübersicht für Iba1 Antikörper (C-Term, Isoform 1) (ABIN374779)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: C-Term, Isoform 1

Sequenz: C-YEEKAREKEK P

Spezifität: This antibody is expected to recognise Isoform 1 (NP_116573.1) and Isoform 3 (NP_001614.3) of AIF1.

Kreuzreaktivität (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Slphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_116573.1, NP_001614.3.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 2 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gen-ID: 199, 9606

UniProt: P55008

Pathways: Smooth Muscle Cell Migration