IL12B Antikörper (Internal Region)

Produktnummer ABIN374765

Der Ziege Polyklonal anti-IL12B Antikörper wird verwendet zum Nachweis von IL12B in Proben von Human. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für IL12B Antikörper (Internal Region) (ABIN374765)

Target: IL12B (Interleukin 12 beta (IL12B))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL12B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-IL12B Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-QGKSKREKKD RVFTD

Spezifität: This antibody recognizes Interleukin-12B (IL12B).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002178.2.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in Human Liver lysates(calculated MW of 37.7 kDa according to NP_002178.2). Immunohistochemistry on Paraffin Embedded Sections: 4-6 μg/mL. In paraffin embedded Human Colon shows cytoplasm staining of individual cells betweencolumns. In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of someDCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Andere Bezeichnung: Interleukin-12 beta / IL12B

Hintergrund: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

Gen-ID: 3593

NCBI Accession: NP_002178

UniProt: P29460

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation