Glutathione Peroxidase 1 Antikörper (Internal Region)

Produktnummer ABIN374697

Dieses Anti-Glutathione Peroxidase 1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Glutathione Peroxidase 1 in WB, IHC (p) und EIA. Geeignet für Human.

Kurzübersicht für Glutathione Peroxidase 1 Antikörper (Internal Region) (ABIN374697)

Target: Glutathione Peroxidase 1 (GPX1)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glutathione Peroxidase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-Glutathione Peroxidase 1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-REALPAPSDD ATA

Spezifität: This antibody is expected to recognise Human Glutathione Peroxidase 1 Isoform 1 only (NP_000572.2).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Syntehtic Peptide from the internal region of Human GPX1 (NP_000572.2). Genename: GPX1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/32000. Western blot: 1-3 μg/mL. Approx 22 kDa band observed in Human Liver and Kidney lysates(calculated MW of 22.2 kDa according to NP_000572.2). Immunohistochemistry: 3.5 μg/mL. In paraffin embedded Human Cerebral Cortex shows staining of microglia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Andere Bezeichnung: Glutathione Peroxidase 1 / GPX1

Hintergrund: Glutathione peroxidases(GPxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. This protein is one of the most important antioxidant enzymes in humans. This protein has been reported to protect cultured breast cancer cells from CD95-induced apoptosis and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. There are several isoforms which differ in their primary structure and localization. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site. One allele of the Glutathione Peroxidase 1 gene is significantly associated with an increased breast cancer risk.Synonyms: Cellular glutathione peroxidase, GSHPx-1

Gen-ID: 2876

NCBI Accession: NP_000572

UniProt: P07203

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom