DARC Antikörper (N-Term)

Produktnummer ABIN374609

Der Ziege Polyklonal anti-DARC Antikörper wird verwendet zum Nachweis von DARC in Proben von Human. Er wurde validiert für WB, FACS und IHC (p).

Kurzübersicht für DARC Antikörper (N-Term) (ABIN374609)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DARC Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognizes Duffy/FY/DARC (N-term).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_002027.2. Genename: DARC

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 35 kDa band observed in human kidney and humanliver lysates (calculated MW of 35.5 kDa according to NP_002027.2). Immunohistochemistry: 3 μg/mL. In paraffin embedded human kidney, stains epithelialcells of renal tubules and endothelial cells of vessels. The granular staining is compatiblewith the endoplasmic reticulum association of the protein. This antibody has also beensuccessfully used on Huamn Lung showing endothelial staining. FACS: A customer has reported Successful staining of HEK293S-TetR transfected with DARCreceptor at a dilution of 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Andere Bezeichnung: CD234 / DARC

Hintergrund: DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system.Synonyms: Duffy antigen/chemokine receptor, FY, Fy glycoprotein, GPD, Glycoprotein D, GpFy, Plasmodium vivax receptor

Gen-ID: 2532, 9606

UniProt: Q16570