PD-L1 Antikörper (C-Term)

Produktnummer ABIN374512

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PD-L1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PD-L1 Antikörper (C-Term) (ABIN374512)

Target: PD-L1 (CD274 (PD-L1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PD-L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PD-L1 Antikörper

Bindungsspezifität: C-Term

Sequenz: CKKQSDTHLE ET

Spezifität: This antibody recognizes Human CD274 / PDL1. Other species not tested.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human (NP_054862.1) Genename: CD274

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western blot: 0.3-1.0 μg/mL. Approx 35 kDa band observed in Human Heart lysates(calculated MW of 33.5 kDa according to NP_054862.1). Immunohistochemistry: 4-6 μg/mL. In paraffin embedded Human Skeletal Muscle shows staining of the striated muscularfibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: CD274 / PDL1

Hintergrund: CD274 [PDL1] is expressed by antigen presenting cells, including human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells. In addition, PDL1 is expressed in nonlymphoid tissues such as heart and lung. The relative levels of inhibitory PDL1 and costimulatory B7 1/B7 2 signals on antigen presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. PDL1 expression on nonlymphoid tissues and its potential interaction with PD1 may subsequently determine the extent of immune responses at sites of inflammation. PDL1 is thought to be involved in fetomaternal tolerance.Synonyms: B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1

Gen-ID: 29126, 9606

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints