Caspase 4 Antikörper (Internal Region)

Produktnummer ABIN374466

Der Ziege Polyklonal anti-Caspase 4 Antikörper (ABIN374466) detektiert spezifisch Caspase 4 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Caspase 4 Antikörper (Internal Region) (ABIN374466)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Caspase 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Caspase 4 Antikörper

Bindungsspezifität: Internal Region

Sequenz: CKERAEEIYP IKERN

Spezifität: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP-150649.1).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 40 kDa band observed in Human Heart lysates(calculated MW of 43.0 kDa according to NP_001216.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Andere Bezeichnung: Caspase-4

Hintergrund: Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67 % homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

Gen-ID: 837, 9606

UniProt: P49662

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity