BIM Antikörper (C-Term)

Produktnummer ABIN374444

Der Ziege Polyklonal anti-BIM Antikörper wird verwendet zum Nachweis von BIM in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für BIM Antikörper (C-Term) (ABIN374444)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BIM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-BIM Antikörper

Bindungsspezifität: C-Term

Sequenz: C-FNAYYARRLE K

Spezifität: This antibody is expected to recognise 3 reported isoforms: BimAD, BimACD and BimABCD.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_619528, NP_996886, NP_619529.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/16,000. Western Blot: 0.5-2 μg/mL. Detects a band of Approx 23 kDa in K562 lysates (PredictedMolecular Weight: 19.9, 12 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Hintergrund: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

Gen-ID: 10018, 9606

UniProt: O43521

Pathways: PI3K-Akt Signalweg, Neurotrophin Signalübertragung, Tube Formation, Positive Regulation of Endopeptidase Activity