ALOX5AP Antikörper (C-Term)

Produktnummer ABIN374441

Der Ziege Polyklonal anti-ALOX5AP Antikörper wird verwendet zum Nachweis von ALOX5AP in Proben von Human. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für ALOX5AP Antikörper (C-Term) (ABIN374441)

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALOX5AP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ALOX5AP Antikörper

Bindungsspezifität: C-Term

Sequenz: C-KTISTTISPL LLIP

Spezifität: This antibody recognizes the C-term of ALOX5AP.
Cellular Localization: Nucleus membrane. Endoplasmic reticulum membrane.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_001620. Genename: ALOX5AP

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32,000. Western Blot: 1 μg/mL. Detects a band of approx 48 kDa (predicted molecular weight: 18 kDa). An explanation has not been found for the difference in the two molecular weightvalues. This band was successfully blocked by incubation with the immunising peptide. Positive Control: Human Brain and U937 lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ALOX5AP

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Andere Bezeichnung: ALOX5AP / FLAP

Hintergrund: Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367], also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.Synonyms: Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein

Gen-ID: 241, 9606

UniProt: P20292