TCP1 alpha/CCTA Antikörper (C-Term)

Produktnummer ABIN374406

Der Ziege Polyklonal anti-TCP1 alpha/CCTA Antikörper (ABIN374406) detektiert spezifisch TCP1 alpha/CCTA in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TCP1 alpha/CCTA Antikörper (C-Term) (ABIN374406)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TCP1 alpha/CCTA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-TCP1 alpha/CCTA Antikörper

Bindungsspezifität: C-Term

Sequenz: C-SYEDAVHSGA LND

Spezifität: This antibody detects CCT1 / TCP1 at C-term. It is expected to recognise both reported isoforms.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1. Genename: TCP1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60 kDa band observed in Hela, Human Lung and HumanTestis lysates (predicted MW of 60 kDa according to NP_110379.2). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows onlycytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining inother germ cells with unstained nucleoli.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Andere Bezeichnung: CCT1 / TCP1

Hintergrund: TCP1 alpha is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.Synonyms: CCT alpha, CCTA, Chaperonin containing T complex polypeptide 1 subunit 1, T-complex protein 1 subunit alpha, TCP-1-alpha, TCP1 alpha

Gen-ID: 6950

NCBI Accession: NP_110379

UniProt: P17987