KPNA4 Antikörper (C-Term)

Produktnummer ABIN374394

Produktdetails anti-KPNA4 Antikörper

Target: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KPNA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-NSSANVPTEG FQF

Spezifität: This antibody detects Importin subunit alpha-4 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002259.1 Genename: KPNA4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55-60 kDa band observed in Human Testis and Ovarylysates (predicted size of approx.50 kDa according to NP_002259.1). Immunohistochemistry: In paraffin embedded Human Testis, shows strong nuclearstaining of some cells in the seminiferous tubules (3 μg/mL). In paraffin embedded HumanSmall Intestine shows pixulate staining in nuclei of enterocytes (1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu KPNA4

Target: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Andere Bezeichnung: KPNA4 / Importin alpha-4 (KPNA4 Produkte)

Synonyme: IPOA3 antikoerper, QIP1 antikoerper, SRP3 antikoerper, 6 antikoerper, ATIMPALPHA3 antikoerper, IMPA-3 antikoerper, IMPORTIN ALPHA 3 antikoerper, IMPORTIN ALPHA ISOFORM 3 antikoerper, MODIFIER OF SNC1 antikoerper, T10M13.16 antikoerper, T10M13_16 antikoerper, LOC100224013 antikoerper, 1110058D08Rik antikoerper, importin antikoerper, ipoa3 antikoerper, qip1 antikoerper, srp3 antikoerper, impa3 antikoerper, wu:fa56d07 antikoerper, wu:fa66g10 antikoerper, wu:fe14c04 antikoerper, wu:fi04h11 antikoerper, wu:fi19b05 antikoerper, karyopherin subunit alpha 4 antikoerper, ARM repeat superfamily protein antikoerper, importin alpha 3 antikoerper, karyopherin (importin) alpha 4 antikoerper, karyopherin alpha 4 (importin alpha 3) S homeolog antikoerper, karyopherin alpha 4 (importin alpha 3) antikoerper, KPNA4 antikoerper, MOS6 antikoerper, LOC100533341 antikoerper, Kpna4 antikoerper, kpna4.S antikoerper, kpna4 antikoerper

Hintergrund: KPNA4 (karyopherin alpha 4), also known as importin alpha 3, is a member of the importin alpha family of proteins which includes KPNA1 (importin alpha 5), KPNA2 (importin alpha 1), KPNA3 (importin alpha 4), KPNA4 (importin alpha 3), KPNA5 (importin alpha 6), and KPNA6 (importin alpha 7). The importin alpha family of proteins plays a role in the import of proteins from the cytoplasm to the nucleus. The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex (NPC) followed by translocation through the nuclear pore. Importin alpha proteins bind the nuclear localization signal (NLS) of cargo proteins and function as adaptor proteins for the nuclear pore receptor KPNB1 (importin beta 1). KPNB1 is responsible for docking the importin-cargo complex to the cytoplasmic side of the NPC for its translocation through the NPC. KPNA3 (Importin alpha 4) and KPNA4 (importin alpha 3) have been reported to mediate the nuclear import of TNF-alpha induced nuclear import of NF-kappaB p50/p65 heterodimers. The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.Synonyms: Importin alpha Q1, Karyopherin subunit alpha-4, QIP1

Gen-ID: 3840

NCBI Accession: NP_002259

UniProt: O00629

Pathways: Protein targeting to Nucleus