POLL Antikörper (C-Term)

Produktnummer ABIN374383

Dieser Ziege Polyklonal Antikörper detektiert spezifisch POLL in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für POLL Antikörper (C-Term) (ABIN374383)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POLL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLL Antikörper

Bindungsspezifität: C-Term

Sequenz: C-LGLPYREPAE RDW

Spezifität: This antibody detects DNA Polymerase Lambda at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037406.1 Genename: POLL

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave a band at approx 40 kDa in Human Liver andHepG2 extracts at 1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Small Intestine showsnuclear staining of enterocytes and of most cells in the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Andere Bezeichnung: DNA Polymerase lambda

Hintergrund: DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.Synonyms: DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda

Gen-ID: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response