NDC80 Antikörper (C-Term)

Produktnummer ABIN374264

Der Ziege Polyklonal anti-NDC80 Antikörper wird verwendet zum Nachweis von NDC80 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für NDC80 Antikörper (C-Term) (ABIN374264)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NDC80 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NDC80 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-YEKKATLIKS SEE

Spezifität: This antibody detects NDC80 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006092.1 Genename: NDC80

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 75-80 kDa band observed in Hela lysate (predicted MWof 70 kDa according to NP_006092).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Andere Bezeichnung: NDC80

Hintergrund: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.Synonyms: HEC, HEC1, KNTC2

Gen-ID: 10403

NCBI Accession: NP_006092

UniProt: O14777

Pathways: Maintenance of Protein Location