RAB11A Antikörper (C-Term)

Produktnummer ABIN374252

Der Ziege Polyklonal anti-RAB11A Antikörper wird verwendet zum Nachweis von RAB11A in Proben von Human, Maus, Ratte und Rind (Kuh). Er wurde validiert für WB.

Kurzübersicht für RAB11A Antikörper (C-Term) (ABIN374252)

Target: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RAB11A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAB11A Antikörper

Bindungsspezifität: C-Term

Sequenz: TENKPKVQCC QNI

Spezifität: This antibody detetcs RAB11A at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Rat, Cow.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_004654.1 Genename: RAB11A

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Mouse Heart lysates (CalculatedMW of 24.6 kDa according to NP_004654.1). In transfected HEK293 transiently expressingRAB11A a band of approx.26 kDa is observed. This band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RAB11A

Target: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Andere Bezeichnung: RAB11A / RAB11

Hintergrund: Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.Synonyms: RAB-11, Ras-related protein Rab-11A, YL8

Gen-ID: 8766

NCBI Accession: NP_004654

UniProt: P62491

Pathways: Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity