Melanophilin Antikörper (C-Term)

Produktnummer ABIN374219

Der Ziege Polyklonal anti-Melanophilin Antikörper wird verwendet zum Nachweis von Melanophilin in Proben von Maus und Ratte. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für Melanophilin Antikörper (C-Term) (ABIN374219)

Target: Melanophilin (MLPH)

Reaktivität: Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Melanophilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-Melanophilin Antikörper

Bindungsspezifität: C-Term

Sequenz: C-ARHIFAKPVM AQQP

Spezifität: This antibody detects Melanophilin at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_443748.2. Genename: Mlph

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80 kDa band observed in Mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high proteinloads. Immunofluorescence: Successfully used at 0.5 μg/mL to stain mouse melanocytes, seepicture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). GST-pull down assay: see publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Melanophilin

Target: Melanophilin (MLPH)

Andere Bezeichnung: Melanophilin

Hintergrund: Melanophilin is a member of the exophilin subfamily of Rab effector proteins. It forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: Exophilin-3, Leaden protein, Ln, Mlph, Slac2a, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a

Gen-ID: 171531

NCBI Accession: NP_443748

UniProt: Q91V27