Iba1 Antikörper (C-Term, Isoform 1)

Produktnummer ABIN374206

Dieser Ziege Polyklonal Antikörper erkennt spezifisch Iba1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus und Schwein.

Kurzübersicht für Iba1 Antikörper (C-Term, Isoform 1) (ABIN374206)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: C-Term, Isoform 1

Spezifität: This antibody is expected to recognizes Isoform 1 (NP_116573.1) and Isoform 3 (NP_001614.3) of AIF1.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Porcine.
Species reactivity (tested):Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3. Genename: AIF1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 16 kDa band observed in Rat Brain lysates (calculatedMW of 16.6 kDa according to Human NP_001614.3 and of 16.6 kDa according to RatNP_058892.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gen-ID: 199

NCBI Accession: NP_116573

UniProt: P55008

Pathways: Smooth Muscle Cell Migration