DKK2 Antikörper (C-Term)
Kurzübersicht für DKK2 Antikörper (C-Term) (ABIN374181)
Target
Alle DKK2 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- C-Term
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Spezifität
- This antibody detects Dickkopf-2 at C-term.
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Kreuzreaktivität (Details)
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Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human. -
Aufreinigung
- Affinity chromatography
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Immunogen
- Peptide with sequence ATYSSKARLHVCQKI, from the C Terminus of the protein sequence Genename: DKK2
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Applikationshinweise
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Peptide ELISA: 1/8000. Western blot: 1 - 3 μg/mL. Approx 28 kDa band observed in Human Heart lysate (calculatedMW of 28.8 kDa according to NP_055236).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 0.5 mg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- DKK2 (Dickkopf 2 Homolog (DKK2))
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Andere Bezeichnung
- Dickkopf-2 (DKK2)
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Hintergrund
- Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.Synonyms: Dickkopf-related protein 2, Dkk-2
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Gen-ID
- 27123
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NCBI Accession
- NP_055236
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UniProt
- Q9UBU2
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Pathways
- WNT Signalweg
Target
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