DKK2 Antikörper (C-Term)

Produktnummer ABIN374181

Dieses Anti-DKK2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von DKK2 in WB. Geeignet für Human, Maus, Ratte, Hund und Rind (Kuh).

Kurzübersicht für DKK2 Antikörper (C-Term) (ABIN374181)

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DKK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DKK2 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects Dickkopf-2 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence ATYSSKARLHVCQKI, from the C Terminus of the protein sequence Genename: DKK2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western blot: 1 - 3 μg/mL. Approx 28 kDa band observed in Human Heart lysate (calculatedMW of 28.8 kDa according to NP_055236).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu DKK2

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Andere Bezeichnung: Dickkopf-2 (DKK2)

Hintergrund: Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.Synonyms: Dickkopf-related protein 2, Dkk-2

Gen-ID: 27123

NCBI Accession: NP_055236

UniProt: Q9UBU2

Pathways: WNT Signalweg