FYN Antikörper (N-Term)

Produktnummer ABIN374105

Produktdetails anti-FYN Antikörper

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FYN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: VQCKDKEATK LTE

Spezifität: This antibody is expected to recognise all three reported isoforms of FYN (NP_002028.1, NP_694592.1 and NP_694593.1).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_002028.1, NP_694592.1, NP_694593.1. Genename: FYN

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55-60 kDa band observed in Jurkat, A431 and NIH-3T3lysates (predicted MW of 60 kDa according to NP_002028.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Andere Bezeichnung: FYN (FYN Produkte)

Synonyme: im:6908851 antikoerper, si:dkey-119m7.5 antikoerper, zgc:109996 antikoerper, c-fyn antikoerper, cfyn-a antikoerper, SLK antikoerper, SYN antikoerper, p59-FYN antikoerper, PKC antikoerper, fyn antikoerper, zgc:86720 antikoerper, AI448320 antikoerper, AW552119 antikoerper, FYN proto-oncogene, Src family tyrosine kinase antikoerper, FYN proto-oncogene, Src family tyrosine kinase S homeolog antikoerper, FYN proto-oncogene, Src family tyrosine kinase b antikoerper, FYN proto-oncogene, Src family tyrosine kinase L homeolog antikoerper, FYN proto-oncogene, Src family tyrosine kinase a antikoerper, Fyn proto-oncogene antikoerper, FYN antikoerper, fyn.S antikoerper, fynb antikoerper, fyn.L antikoerper, Fyn antikoerper, fyna antikoerper

Hintergrund: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

Gen-ID: 2534

NCBI Accession: NP_002028

UniProt: P06241

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling