B-Cell Linker Antikörper (C-Term)

Produktnummer ABIN374092

Dieser Ziege Polyklonal Antikörper detektiert spezifisch B-Cell Linker in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schwein.

Kurzübersicht für B-Cell Linker Antikörper (C-Term) (ABIN374092)

Target: B-Cell Linker (BLNK)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser B-Cell Linker Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-B-Cell Linker Antikörper

Bindungsspezifität: C-Term

Sequenz: C-KDSTRLKYAV KVS

Spezifität: Detects B-cell linker protein/BLNK at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1 Genename: BLNK

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: BLNK antibody Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in lysates of cell line Daudi(calculated MW of 50.0 kDa according to 037446.1). The observed molecular weightcorresponds to how SLP-65 is expected to run. However, when transiently expressed inHEK293, a 50 kDa band is observed.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: B-Cell Linker Protein / BLNK

Hintergrund: BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.Synonyms: B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa

Gen-ID: 29760

NCBI Accession: NP_037446

UniProt: Q8WV28

Pathways: BCR Signaling