NCF1 Antikörper (C-Term)

Produktnummer ABIN374079

Produktdetails anti-NCF1 Antikörper

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NCF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-SESTKRKLAS AV

Spezifität: This antibody detects NCF-1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Rabbit, Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein according to NP_000256.3. Genename: NCF1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 20000. Western blot: 0.2-2 μg/mL. Approx 47 kDa band seen in U937 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: NCF1 (NCF1 Produkte)

Synonyme: NCF1A antikoerper, NOXO2 antikoerper, SH3PXD1A antikoerper, p47phox antikoerper, Ncf-1 antikoerper, p47 antikoerper, neutrophil cytosolic factor 1 antikoerper, NCF1 antikoerper, Ncf1 antikoerper

Hintergrund: NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NOXO2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, SH3PXD1A, p47-phox

Gen-ID: 653361

NCBI Accession: NP_000256

UniProt: P14598

Pathways: PI3K-Akt Signalweg