MELK Antikörper (C-Term)

Produktnummer ABIN374073

Der Ziege Polyklonal anti-MELK Antikörper (ABIN374073) detektiert spezifisch MELK in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Rind (Kuh) und Hund.

Kurzübersicht für MELK Antikörper (C-Term) (ABIN374073)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reaktivität: Human, Maus, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MELK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-MELK Antikörper

Bindungsspezifität: C-Term

Sequenz: KRLVEDILSS CKV

Spezifität: This antibody detects MELK at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Canine (Dog), Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Syntehtic Peptide from the C Terminus of the protein sequence according to NP_055606.1. Genename: MELK

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3´μg/mL. Experiments gave bands at approx 75 kDa and 150 kDa inhuman testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The 150 kDa band is explained by glycosylation of this member of the AMPK family. Thisprotein has a calculated MW of 74.6 kDa according to NP_055606.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containng 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Andere Bezeichnung: MELK

Hintergrund: MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine. MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK

Gen-ID: 9833

NCBI Accession: NP_055606

UniProt: Q14680