DDB1 Antikörper (C-Term)

Produktnummer ABIN374069

Es gibt 1 Publikation für dieses Produkt. Der Ziege Polyklonal anti-DDB1 Antikörper wird verwendet zum Nachweis von DDB1 in Proben von Human, Maus, Ratte, Hund und Rind (Kuh). Er wurde validiert für WB und EIA.

Kurzübersicht für DDB1 Antikörper (C-Term) (ABIN374069)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-DDB1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-DLIKVVEELT RIH

Spezifität: This antibody detects DNA damage-binding protein 1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein Genename: DDB1

Anwendungsinformationen

Applikationshinweise: ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 130 kDa band seen in HeLa, Jurkat and NSO (mouse)cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-DDB1 Antikörper (ABIN374069)

Leung-Pineda, Huh, Piwnica-Worms: "DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress." in: Cancer research, Vol. 69, Issue 6, pp. 2630-7, (2009) (PubMed).

Details zu DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Andere Bezeichnung: DDB1 / XAP1

Hintergrund: Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein

Gen-ID: 1642

NCBI Accession: NP_001914

UniProt: Q16531

Pathways: DNA Reparatur