PDGFRA Antikörper (C-Term)

Produktnummer ABIN372897

Der Kaninchen Polyklonal anti-PDGFRA Antikörper (ABIN372897) detektiert spezifisch PDGFRA in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für PDGFRA Antikörper (C-Term) (ABIN372897)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PDGFRA Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody is specific for PDGFR alpha and does not react with PDGFR beta.
Cellular Localization: Cytoplasmic and cell membrane.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: A synthetic peptide derived from C-terminal of Human PDGFR alpha.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 μg/mL for 2 hrs at RT. Immunohistochemistry on Paraffin Sections: 5 μg/mL for 45 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for10-20 min followed by cooling at RT for 20 min. Positive Control: Ovarian carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.6, 0.2 % BSA, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: CD140a / PDGFRA

Hintergrund: Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

Gen-ID: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling