Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
This antibody recognizes Calcium/Calmodulin-dependent Protein Kinase Type II (CAMK2). Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment.
Kreuzreaktivität (Details)
Species reactivity (expected):Human, Mouse, Xenopus. Species reactivity (tested):Rat.
Aufreinigung
Immunoaffinity Chromatography
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in Rat brain CaM Kinase II.
CAMK2A
Reaktivität: Human
ELISA, IHC, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunohistochemistry on paraffin sections: 1/200. Western blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
camk2 antikoerper, camk2b antikoerper, zgc:112538 antikoerper, zgc:123320 antikoerper, CAMKA antikoerper, CaMKII antikoerper, R74975 antikoerper, mKIAA0968 antikoerper, PK2CDD antikoerper, PKCCD antikoerper, calcium/calmodulin dependent protein kinase II alpha antikoerper, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antikoerper, calcium/calmodulin-dependent protein kinase II alpha antikoerper, CAMK2A antikoerper, camk2a.S antikoerper, camk2a antikoerper, Camk2a antikoerper
Hintergrund
CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/Calmodulin-dependent Protein Kinase Type II, KIAA0968