Cathepsin K Antikörper

Produktnummer ABIN372829

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cathepsin K in WB, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Schwein, Kaninchen und Huhn.

Kurzübersicht für Cathepsin K Antikörper (ABIN372829)

Target: Cathepsin K (CTSK)

Reaktivität: Human, Ratte, Maus, Schwein, Kaninchen, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin K Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cathepsin K Antikörper

Spezifität: The antibody detects proform (43 kDa) and the mature form (29 kDa) of Cathepsin K.

Kreuzreaktivität (Details): Species reactivity (expected):Chicken, Human, Mouse, Porcine, Rabbit.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide surrounding amino acid 321 of Rat Cathepsin K .

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-4 μg/mL. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Cathepsin K

Target: Cathepsin K (CTSK)

Andere Bezeichnung: Cathepsin K

Hintergrund: Cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin K expression is highest in bone, cartilage, and skeletal muscle. Cathepson K is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in the gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature.Synonyms: CTSK, CTSO, CTSO2, Cathepsin O, Cathepsin O2, Cathepsin X

Gen-ID: 1513

NCBI Accession: NP_000387

UniProt: P43235

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades