JAG1 Antikörper (AA 110-125)

Produktnummer ABIN372790

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch JAG1 in WB, IF, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Schimpanse.

Kurzübersicht für JAG1 Antikörper (AA 110-125) (ABIN372790)

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Human, Maus, Ratte, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-JAG1 Antikörper

Bindungsspezifität: AA 110-125

Spezifität: Recognizes Human Jagged-1 (JAG1).

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Rat and Mouse (100 % homology).Canine (81 %) and Xenopus laevis (85 %).
Species reactivity (tested):Human.

Aufreinigung: Protein A Chromatography.

Immunogen: Synthetic peptide corresponding to aa 110-125 of Human Jagged-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2500-1/10000. Western Blot: 1/250-1/1000. Detects a band approximately 150 kD in size corresponding toJagged-1 in Mouse liver whole cell lysate and a 70 kD band in Human brain and kidneylysates. Immunocytochemistry. Immunofluorescence: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.53 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: CD339 / JAG1

Hintergrund: Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.Synonyms: JAGL1, Jagged-1, hJ1

Gen-ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance