Tryptophan Hydroxylase 1 Antikörper (pSer58)

Produktnummer ABIN372732

Der Kaninchen Polyklonal anti-Tryptophan Hydroxylase 1 Antikörper (ABIN372732) detektiert spezifisch Tryptophan Hydroxylase 1 in WB und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Kaninchen, Huhn, Rind (Kuh), Xenopus laevis und Zebrafisch (Danio rerio).

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (pSer58) (ABIN372732)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Human, Maus, Ratte, Kaninchen, Huhn, Rind (Kuh), Xenopus laevis, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Bindungsspezifität: pSer58

Spezifität: Specific for the ~53k Tryptophan Hydroxylase protein phosphorylated at Ser58.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Human, Mouse, Rat, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rabbit.

Aufreinigung: Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat Tryptophan Hydroxylase 1 (TPH1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan 5-Hydroxylase 1 (TPH1)

Hintergrund: Tryptophan Hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

Gen-ID: 100009100

NCBI Accession: NP_001075741

UniProt: P17290

Pathways: Feeding Behaviour