Tryptophan Hydroxylase 1 Antikörper (pSer58)

Produktnummer ABIN372732

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Target: Tryptophan Hydroxylase 1 (TPH1)

Bindungsspezifität: pSer58

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Huhn, Zebrafisch (Danio rerio), Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Specific for the ~53k Tryptophan Hydroxylase protein phosphorylated at Ser58.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Human, Mouse, Rat, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rabbit.

Aufreinigung: Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat Tryptophan Hydroxylase 1 (TPH1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan 5-Hydroxylase 1 (TPH1) (TPH1 Produkte)

Synonyme: TPRH antikoerper, TRPH antikoerper, tph antikoerper, tph1 antikoerper, tphD1 antikoerper, tph2 antikoerper, tph1l antikoerper, tphD2 antikoerper, Tph antikoerper, TPH antikoerper, SERGEF antikoerper, LOC100229598 antikoerper, tryptophan hydroxylase 1 antikoerper, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a antikoerper, TryPtophan Hydroxylase antikoerper, tryptophan hydroxylase 1b antikoerper, tryptophan hydroxylase 1 L homeolog antikoerper, tryptophan 5-hydroxylase 1 antikoerper, TPH1 antikoerper, tph1a antikoerper, tph-1 antikoerper, tph1b antikoerper, Tph1 antikoerper, tph1.L antikoerper, LOAG_02149 antikoerper, Tsp_05203 antikoerper

Hintergrund: Tryptophan Hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

Gen-ID: 100009100

NCBI Accession: NP_001075741

UniProt: P17290

Pathways: Feeding Behaviour