Ephrin B1 Antikörper (pTyr331)

Produktnummer ABIN372621

Dieses Anti-Ephrin B1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Ephrin B1 in WB. Geeignet für Human, Maus, Ratte, Huhn, Rind (Kuh), Zebrafisch (Danio rerio) und Xenopus laevis.

Kurzübersicht für Ephrin B1 Antikörper (pTyr331) (ABIN372621)

Target: Ephrin B1 (EFNB1)

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Zebrafisch (Danio rerio), Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ephrin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Ephrin B1 Antikörper

Bindungsspezifität: pTyr331

Spezifität: Specific for the ~46k Ephrin-B protein phosphorylated at Tyr331. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Chicken, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of Chicken Ephrin-B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Ephrin B1

Target: Ephrin B1 (EFNB1)

Andere Bezeichnung: Ephrin-B1

Hintergrund: Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2

Gen-ID: 395896

NCBI Accession: NP_990366

UniProt: O73612

Pathways: RTK Signalweg