Ephrin B1 Antikörper (pTyr298)

Produktnummer ABIN372619

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Ephrin B1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Huhn, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Xenopus laevis und Non-Human Primate.

Kurzübersicht für Ephrin B1 Antikörper (pTyr298) (ABIN372619)

Target: Ephrin B1 (EFNB1)

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Xenopus laevis, Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ephrin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Ephrin B1 Antikörper

Bindungsspezifität: pTyr298

Spezifität: Specific for the ~46k Ephrin-B protein phosphorylated at Tyr298. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr298 of Xenopus Ephrin-B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Ephrin B1

Target: Ephrin B1 (EFNB1)

Andere Bezeichnung: Ephrin-B1

Hintergrund: Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2

Gen-ID: 394292

NCBI Accession: NP_001080948

UniProt: O13097

Pathways: RTK Signalweg