Collagen Type I Antikörper

Produktnummer ABIN372600

Produktdetails anti-Collagen Type I Antikörper

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human, Maus, Säugetier, Fisch, Schaf, Amphibien, Avian

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Specific for the ~140 kDa telopeptide portion of the Collagen I Alpha-1 polypeptide.

Kreuzreaktivität (Details): Species reactivity (expected):all Mammals, Birds and Amphibians.
Species reactivity (tested):Human, Mouse, Rat and Sheep.

Aufreinigung: Affinity Chromatography.

Immunogen: Peptide from the Human Collagen I Alpha-1 telopeptide sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody works well for Immunohistochemistry on Paraformaldehyde-Fixed Sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mMsodium citrate ( pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections ofFormaldehyde-Fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen Ithat has formed fibrils in the extracellular matrix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type I (COL1 Produkte)

Synonyme: collagen type III alpha 1 chain antikoerper, COL3A1 antikoerper

Hintergrund: Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gen-ID: 1277

NCBI Accession: NP_000079

UniProt: P02452