GCLC Antikörper (AA 295-313)
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- Target Alle GCLC Antikörper anzeigen
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
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Bindungsspezifität
- AA 295-313
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Reaktivität
- Human, Maus, Ratte, Rind (Kuh), Affe
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GCLC Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- Recognizes the heavy subunit of GCS.
- Kreuzreaktivität (Details)
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Species reactivity (expected):Human, Monkey, Mouse and Rat, Bovine (weak).
Species reactivity (tested):Rat. - Aufreinigung
- Protein A Chromatography.
- Immunogen
- Synthetic peptide derived from corresponding to aa 295-313 of Rat GCLC (heavy subunit of GCS).
- Isotyp
- IgG
- Top Product
- Discover our top product GCLC Primärantikörper
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- Applikationshinweise
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ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 10 mM PBS, pH 7.4 containing 0.09 % Sodium Azide as presrevtaive and 40 % Glycerol, 0.2 % BSA as stabilizers..
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (Add 40-50% glycerol) at-20 °C for longer.
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- Target
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Andere Bezeichnung
- GCLC / GLCLC (GCLC Produkte)
- Synonyme
- GCLC antikoerper, gclc antikoerper, MGC146287 antikoerper, 2259 antikoerper, CG2259 antikoerper, DmGCLC antikoerper, DmGCS antikoerper, DmGCSh antikoerper, Dmel\\CG2259 antikoerper, GCL antikoerper, GCLc antikoerper, GCSh antikoerper, Gcs antikoerper, Gcsh antikoerper, DDBDRAFT_0186120 antikoerper, DDBDRAFT_0231403 antikoerper, DDB_0186120 antikoerper, DDB_0231403 antikoerper, GCS antikoerper, GLCL antikoerper, GLCLC antikoerper, D9Wsu168e antikoerper, GLCL-H antikoerper, Ggcs-hs antikoerper, Glclc antikoerper, cb1049 antikoerper, fe36e11 antikoerper, wu:fe36e11 antikoerper, glutamate-cysteine ligase catalytic subunit antikoerper, glutamate-cysteine ligase, catalytic subunit antikoerper, Glutamate-cysteine ligase catalytic subunit antikoerper, gamma glutamylcysteine synthetase antikoerper, glutamate-cysteine ligase, catalytic subunit L homeolog antikoerper, glutamate-cysteine ligase, catalytic subunit S homeolog antikoerper, gamma-glutamylcysteine synthetase antikoerper, GCLC antikoerper, gclc antikoerper, Gclc antikoerper, LbGCS antikoerper, gcsA antikoerper, gclc.L antikoerper, gclc.S antikoerper, GSH1 antikoerper
- Hintergrund
- Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2 mM and increases the inhibitory constant for GSH.Synonyms: GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Gamma-glutamylcysteine synthetase, Glutamate-cysteine ligase catalytic subunit
- Gen-ID
- 2729
- NCBI Accession
- NP_001184044
- UniProt
- P48506
- Pathways
- Cell RedoxHomeostasis
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