IFIH1 Antikörper (C-Term)

Produktnummer ABIN372470

Der Kaninchen Polyklonal anti-IFIH1 Antikörper wird verwendet zum Nachweis von IFIH1 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für IFIH1 Antikörper (C-Term) (ABIN372470)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFIH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IFIH1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody recognizes Melanoma Differentiation Associated Protein-5 (MDA5/IFIH1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to 16 amino acids from near the carboxy terminus of human MDA5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Andere Bezeichnung: Helicard

Hintergrund: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents, treatment with either agent alone only achieves reversible differentiation. It is believed that MDA-5 may function to promote message degradation by specific RNases. It also seems to possess growth inhibition properties. The interaction of MDA5 with double stranded RNA can lead to induction of IFN required for the the innate immune response against viruses.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116

Gen-ID: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response