LSD1 Antikörper (AA 100-150)

Produktnummer ABIN372401

Der Kaninchen Polyklonal anti-LSD1 Antikörper wird Verwendung zum Nachweis von LSD1 in proben von Human, Maus, Ratte, Affe, Hund, Makake und Zebrafisch (Danio rerio). Er wurde validiert für WB und IHC (p).

Kurzübersicht für LSD1 Antikörper (AA 100-150) (ABIN372401)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reaktivität: Human, Maus, Ratte, Affe, Hund, Makake, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LSD1 Antikörper

Bindungsspezifität: AA 100-150

Spezifität: This antibody recognizes Amine Oxidase (Flavin Containing) Domain 2 (AOF2/KDM1A).

Kreuzreaktivität (Details): Species reactivity (expected):Dog (Canine), Macaque, Monkey, Mouse, Rat and Zebrafish.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a portion of amino acids of human AOF2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 0.5-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Andere Bezeichnung: AOF2

Hintergrund: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.Synonyms: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1

Molekulargewicht: 93 kDa.

Gen-ID: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effekt