Progesterone Receptor Antikörper (pSer294)

Produktnummer ABIN371835

Dieses Anti-Progesterone Receptor-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Progesterone Receptor in WB und IHC (fro). Geeignet für Human, Ratte, Maus und Non-Human Primate.

Kurzübersicht für Progesterone Receptor Antikörper (pSer294) (ABIN371835)

Target: Progesterone Receptor (PGR)

Reaktivität: Human, Ratte, Maus, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Progesterone Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 608

Produktdetails anti-Progesterone Receptor Antikörper

Bindungsspezifität: pSer294

Spezifität: This antibody is specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser294. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and non-Human Primates.
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: PR, PGR, NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser294of Human progesterone receptor.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000. Immunohistochemitry (Frozen Sections, unpublished observations): 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Progesterone Receptor

Target: Progesterone Receptor (PGR)

Andere Bezeichnung: Progesterone Receptor

Hintergrund: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000, Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.Synonyms: NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR

Gen-ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration