LAMP1 Antikörper

Produktnummer ABIN371199

Dieses Anti-LAMP1-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von LAMP1 in WB, FACS, IP, IHC (fro) und IEM. Geeignet für Maus.

Kurzübersicht für LAMP1 Antikörper (ABIN371199)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Klon: 1D4B

Produktdetails anti-LAMP1 Antikörper

Spezifität: This antibody is specific to CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa).

Kreuzreaktivität (Details): Species reactivity (tested):Mouse

Produktmerkmale: Synonyms: LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, CD107Antigen-like Family Member A

Aufreinigung: Purified

Immunogen: Plasma membrane fraction of mouse embryo NIH3T3 cell line. (Ref. 1)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: < / = 1 μg/10^6 cells. (Ref.5)Immunohistochemistry on Frozen Sections. (Ref.5)Immunoaffinity Chromatography. (Ref.2)Western blot. (Ref.5)Immunoprecipitation. (Ref.1,3)Immunoelectron Microscopy. (Ref.6)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: CD107a / LAMP1

Hintergrund: CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. (Ref.1) It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. (Ref.2) CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. (Ref.3) LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. (Ref.4)Synonyms: CD107 Antigen-like Family Member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1

Gen-ID: 16783

UniProt: P11438

Pathways: Autophagie