F12 Antikörper

Produktnummer ABIN364435

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch F12 in ELISA, IHC, IP, IHC (p) und IEP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für F12 Antikörper (ABIN364435)

Target: F12 (Coagulation Factor XII (F12))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser F12 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectrophoresis (IEP)

Produktdetails anti-F12 Antikörper

Marke: IHC-plus™

Spezifität: Specific for Factor XII as demonstrated by immunodiffusion. A single precipitin arc was obtained with normal plasma and no reaction was observed with F. XII-deficient plasma.

Aufreinigung: Ammonium sulfate precipitation

Immunogen: Activated human Factor XII (F XIIa) purified from plasma.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IE, IHC, IHC-P (5 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.4, 0.15 M sodium chloride, 50 % glycerol. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu F12

Target: F12 (Coagulation Factor XII (F12))

Andere Bezeichnung: F12 / Factor XII

Hintergrund: Name/Gene ID: F12
Subfamily: Serine S1
Family: Protease

Synonyms: F12, Beta-factor XIIa part 2, Beta-factor XIIa part 1, Coagulation factor XII, F12 deficiency, Factor XII, Factor XII deficiency, HAE3, HAEX, Hageman factor, HAF

Gen-ID: 2161

UniProt: P00748

Pathways: Komplementsystem