Leptin Receptor Antikörper (AA 829-841)

Produktnummer ABIN364407

Dieses Anti-Leptin Receptor-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Leptin Receptor in WB, ELISA, IHC und IHC (p). Geeignet für Human.

Kurzübersicht für Leptin Receptor Antikörper (AA 829-841) (ABIN364407)

Target: Leptin Receptor (LEPR)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Leptin Receptor Antikörper

Bindungsspezifität: AA 829-841

Marke: IHC-plus™

Spezifität: Human LEPR / Leptin Receptor. This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Marmoset, Panda, Dog, Horse (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-TQDDIEKHQSDAG from an internal region of human LEPR / Leptin Receptor (NP_002294.2, NP_001003679.1, NP_001003680.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Marmoset, Panda, Dog, Horse (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: LEPR / Leptin Receptor

Hintergrund: Name/Gene ID: LEPR

Synonyms: LEPR, CD295 antigen, HuB219, LEP-R, OB receptor, LEPRD, OB-R, OBR, CD295, DB, Leptin receptor

Gen-ID: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

UniProt: P48357

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour