HMGA1 Antikörper (AA 12-23)

Produktnummer ABIN364402

Dieser Ziege Polyklonal Antikörper detektiert spezifisch HMGA1 in IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Hund, Schwein, Pferd, Kaninchen, Fledermaus, Hamster und Affe.

Kurzübersicht für HMGA1 Antikörper (AA 12-23) (ABIN364402)

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reaktivität: Human, Ratte, Maus, Hund, Schwein, Pferd, Kaninchen, Fledermaus, Hamster, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HMGA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HMGA1 Antikörper

Bindungsspezifität: AA 12-23

Spezifität: Human HMGA1. This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Horse, Rabbit, Pig (100%) Gibbon, Bovine, Opossum (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-LASKQEKDGTEK from an internal region of human HMGA1 (NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Horse, Rabbit, Pig (100%), Gibbon, Bovine, Opossum (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Andere Bezeichnung: HMGIY / HMGA1

Hintergrund: Name/Gene ID: HMGA1

Synonyms: HMGA1, High mobility group protein A1, High mobility group protein R, HMG-R, Hmg-i, HMGIY, High mobility group AT-hook 1, HMG-I(Y), HMGA1A

Gen-ID: 3159

NCBI Accession: NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910

Pathways: Nuclear Hormone Receptor Binding, Autophagie