Sec8 Antikörper

Produktnummer ABIN364381

Dieser Maus Monoklonal Antikörper erkennt spezifisch Sec8 in WB, IHC, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schwein, Hund, Huhn, Affe, Hamster, Meerschweinchen, Schaf und Forelle.

Kurzübersicht für Sec8 Antikörper (ABIN364381)

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schwein, Hund, Huhn, Affe, Hamster, Meerschweinchen, Schaf, Forelle

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Sec8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 14G1

Produktdetails anti-Sec8 Antikörper

Spezifität: Detects an ~110 kD protein, corresponding to the apparent molecular mass of rSec8 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout) guinea pig, hamster, monkey, pig, rabbit and sheep origins.

Aufreinigung: Protein G purified

Immunogen: Recombinant rat rSec8 (his-tagged) expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), IP (12.5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Konservierungsmittel: PMSF

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Sec8

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Andere Bezeichnung: EXOC4 / SEC8

Hintergrund: Name/Gene ID: EXOC4

Synonyms: EXOC4, Exocyst complex component Sec8, Exocyst complex component 4, KIAA1699, Sec8p, SEC8-like 1 (S. cerevisiae), SEC8, SEC8-like 1, SEC8L1

Gen-ID: 60412

UniProt: Q96A65

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis