LC3B Antikörper (N-Term)

Produktnummer ABIN363542

Der Kaninchen Polyklonal Anti-LC3B-Antikörper wurde für WB, IHC, IF, FACS, ICC und IEM validiert. Er ist geeignet, LC3B in Proben von Human, Maus, Ratte und Rind (Kuh) zu detektieren.

Kurzübersicht für LC3B Antikörper (N-Term) (ABIN363542)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Produktdetails anti-LC3B Antikörper

Bindungsspezifität: N-Term

Spezifität: Reacts with LC3B from human, mouse, rat and bovine and Zebrafish

Kreuzreaktivität (Details): Other species have not been tested.

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide made to the N-terminal region of the human LC3, isoform B protein

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF(1:2000) FC (1:200) IHC (1:400) WB (1:1000)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: Light Chain 3 beta (LC3B)

Hintergrund: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gen-ID: 81631

Pathways: Autophagie