MAP1LC3A Antikörper (AA 50-150)

Produktnummer ABIN363516

Dieses Anti-MAP1LC3A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MAP1LC3A in WB, IF, IHC (p), ICC und IP. Geeignet für Human, Maus, Ratte und Zebrafisch (Danio rerio).

Kurzübersicht für MAP1LC3A Antikörper (AA 50-150) (ABIN363516)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP1LC3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-MAP1LC3A Antikörper

Bindungsspezifität: AA 50-150

Spezifität: Reacts with LC3 from human, mouse, rat and Zebrafish

Kreuzreaktivität: Rind (Kuh)

Kreuzreaktivität (Details): Predicted to react with Xenopus and bovine based on 100 % sequence homology.

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Andere Bezeichnung: Light Chain 3 (LC3)

Hintergrund: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gen-ID: 84557

Pathways: Autophagie