MAP1LC3A Antikörper (AA 50-150)

Produktnummer ABIN363516

Dieser Anti-MAP1LC3A Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von MAP1LC3A in WB, IF, ICC, IP und IHC (p). Geeignet für Human, Maus, Ratte und Zebrafisch (Danio rerio).

Kurzübersicht für MAP1LC3A Antikörper (AA 50-150) (ABIN363516)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP1LC3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MAP1LC3A Antikörper

Bindungsspezifität: AA 50-150

Spezifität: Reacts with LC3 from human, mouse, rat and Zebrafish

Kreuzreaktivität: Rind (Kuh)

Kreuzreaktivität (Details): Predicted to react with Xenopus and bovine based on 100 % sequence homology.

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 50-150)

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:100-1:300, IHC1:200-1:400, IHC-P1:200-1:400, IP20 μL / 500 μg of lysate, WB2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Andere Bezeichnung: Light Chain 3 (LC3)

Hintergrund: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gen-ID: 84557

Pathways: Autophagie