Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
The antibody detects endogenous level of total Histone H2A.X protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 137-141 (Q-A-S-Q-E) derived from Human Histone H2A.X. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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Li, Hu, He, Li, Zhu, Hou, Zhang, Li, Zhang, Wang, Liu, Yao, Zhang: "Up-regulation of NDRG2 through nuclear factor-kappa B is required for Leydig cell apoptosis in both human and murine infertile testes." in: Biochimica et biophysica acta, Vol. 1822, Issue 2, pp. 301-13, (2012) (PubMed).
Li, Kikuchi, Zheng, Noguchi, Takahashi, Nishida, Masuda, Yang, Takano: "Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma." in: Oral oncology, Vol. 47, Issue 3, pp. 217-23, (2011) (PubMed).
Li, Nishida, Noguchi, Zheng, Takahashi, Yang, Masuda, Takano: "Decreased nuclear expression and increased cytoplasmic expression of ING5 may be linked to tumorigenesis and progression in human head and neck squamous cell carcinoma." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 10, pp. 1573-83, (2010) (PubMed).
H2A.X antikoerper, H2A/X antikoerper, H2AX antikoerper, AW228881 antikoerper, H2ax antikoerper, Hist5-2ax antikoerper, gammaH2ax antikoerper, zgc:56329 antikoerper, h2a.x antikoerper, h2a/x antikoerper, h2ax antikoerper, RGD1566119 antikoerper, h2a antikoerper, h2afx antikoerper, H2A histone family member X antikoerper, H2A histone family, member X antikoerper, H2A histone family member X L homeolog antikoerper, histone cluster 1, H2ah antikoerper, histone cluster 2, H2ab S homeolog antikoerper, histone H2AX antikoerper, H2AFX antikoerper, H2afx antikoerper, h2afx antikoerper, h2afx.L antikoerper, HIST1H2AH antikoerper, hist2h2ab.S antikoerper, LOC100522201 antikoerper, LOC100720536 antikoerper
Hintergrund
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation