Cortactin Antikörper (pTyr466)

Produktnummer ABIN362346

Der Kaninchen Polyklonal anti-Cortactin Antikörper wird verwendet zum Nachweis von Cortactin in Proben von Human und Maus. Er wurde validiert für WB.

Kurzübersicht für Cortactin Antikörper (pTyr466) (ABIN362346)

Target: Cortactin (CTTN)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cortactin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Cortactin Antikörper

Bindungsspezifität: pTyr466

Spezifität: The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr466 (P-V-Y (p) -E-T) derived from Human CORTACTIN. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: Cortactin

Hintergrund: Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Molekulargewicht: 85 kDa

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: MAPK Signalweg