GRP78 Antikörper

Produktnummer ABIN361825

Dieses Anti-GRP78-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GRP78 in WB, IHC, IF, ICC und IP. Geeignet für Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für GRP78 Antikörper (ABIN361825)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-GRP78 Antikörper

Spezifität: Detects ~78 kDa.

Kreuzreaktivität: Rind (Kuh), Hund, Fusarium, Hamster, Human, Affe, Maus, Kaninchen, Ratte, Xenopus laevis

Aufreinigung: Peptide Affinity Purified

Immunogen: Rat GRP78 (Bip) synthetic peptide conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN361824 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-GRP78 Antikörper (ABIN361825)

Guess, Ayoob, Chanley, Manley, Cajaiba, Agrawal, Pengal, Pyle, Becknell, Kopp, Ronkina, Gaestel, Benndorf, Smoyer: "Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis." in: PLoS ONE, Vol. 8, Issue 1, pp. e54239, (2013) (PubMed).

Krivoruchko, Storey: "Activation of the unfolded protein response during anoxia exposure in the turtle Trachemys scripta elegans." in: Molecular and cellular biochemistry, Vol. 374, Issue 1-2, pp. 91-103, (2013) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: GRP78

Hintergrund: GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Gen-ID: 25617

NCBI Accession: NP_037215

UniProt: P06761

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling