Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

LAMP2 Antikörper

Der Ratte Monoklonal Anti-LAMP2-Antikörper wurde für WB, IP, ICC und IF validiert. Er ist geeignet, LAMP2 in Proben von Maus zu detektieren.
Produktnummer ABIN361746

Kurzübersicht für LAMP2 Antikörper (ABIN361746)

Target

Alle LAMP2 Antikörper anzeigen
LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität

  • 122
  • 77
  • 38
  • 16
  • 4
  • 3
  • 2
  • 1
Maus

Wirt

  • 97
  • 40
  • 35
Ratte

Klonalität

  • 90
  • 81
Monoklonal

Konjugat

  • 74
  • 20
  • 17
  • 13
  • 9
  • 6
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser LAMP2 Antikörper ist unkonjugiert

Applikation

  • 115
  • 59
  • 59
  • 48
  • 43
  • 32
  • 27
  • 14
  • 13
  • 13
  • 11
  • 7
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon

GL2A7
  • Spezifität

    Detects ~100-110 kDa.

    Kreuzreaktivität

    Human, Maus, Kaninchen

    Aufreinigung

    Protein G Purified

    Immunogen

    Purified preparation of mouse liver lysosomal membranes

    Isotyp

    IgG1
  • Applikationshinweise

    • WB (1:1000)
    • ICC/IF (1:500)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    1 μg/ml of ABIN361745 was sufficient for detection of LAMP2 in 20 μg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    -20°C
  • Target

    LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

    Andere Bezeichnung

    LAMP2

    Hintergrund

    Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

    Gen-ID

    16784

    NCBI Accession

    NP_001017959

    UniProt

    P17047

    Pathways

    Autophagie
Sie sind hier:
Chat with us!