DNAJB1 Antikörper

Produktnummer ABIN361662

Der Maus Monoklonal Anti-DNAJB1-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, DNAJB1 in Proben von Saccharomyces cerevisiae zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für DNAJB1 Antikörper (ABIN361662)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNAJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 2A7-H6

Produktdetails anti-DNAJB1 Antikörper

Spezifität: Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.

Kreuzreaktivität: Saccharomyces cerevisiae

Aufreinigung: Protein G Purified

Immunogen: Full length protein HSP40 (YDJ1)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-DNAJB1 Antikörper (ABIN361662)

Knighton, Nitika, Waller, Strom, Wolfgeher, Reitzel, Truman: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." in: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).

Sluder, Nitika, Knighton, Truman: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." in: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).

Details zu DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Andere Bezeichnung: HSP40

Hintergrund: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

Gen-ID: 855661

NCBI Accession: NP_014335

UniProt: P25491